产品描述

This Human EFEMP1 ELISA Kit was designed for the quantitative measurement of Human EFEMP1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

别名

DHRD ELISA Kit; Doyne honeycomb retinal degeneration ELISA Kit; DRAD ELISA Kit; EFEMP 1 ELISA Kit; EFEMP1 ELISA Kit; EGF containing fibulin like extracellular matrix protein 1 ELISA Kit; EGF containing fibulin like extracellular matrix protein 1 precursor ELISA Kit; EGF-containing fibulin-like extracellular matrix protein 1 ELISA Kit; Epidermal growth factor containing fibulin like extracellular matrix protein 1 ELISA Kit; Extracellular protein S1 5 ELISA Kit; Extracellular protein S1-5 ELISA Kit; FBLN 3 ELISA Kit; FBLN3 ELISA Kit; FBLN3_HUMAN ELISA Kit; FBNL ELISA Kit; FIBL 3 ELISA Kit; FIBL-3 ELISA Kit; FIBL3 ELISA Kit; Fibrillin like ELISA Kit; Fibrillin like protein ELISA Kit; Fibrillin-like protein ELISA Kit; FIBULIN 3 ELISA Kit; Fibulin-3 ELISA Kit; Fibulin3 ELISA Kit; FLJ35535 ELISA Kit; Heat shock 70 KD protein 1 ELISA Kit; MGC111353 ELISA Kit; MLVT ELISA Kit; MTLV ELISA Kit; S1 5 ELISA Kit; T16 protein ELISA Kit

缩写

EFEMP1

Uniprot No

Q12805

灵敏度

0.39 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Neuroscience

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human EFEMP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		102
		Range %		98-109
1:2		Average %		84
		Range %		80-88
1:4		Average %		90
		Range %		85-94
1:8		Average %		93
		Range %		89-97

回收率

The recovery of human EFEMP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		89		83-94
EDTA plasma (n=4)		95		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 100 2.261 2.127 2.194 2.074 50 1.539 1.500 1.520 1.400 25 0.854 0.902 0.878 0.758 12.5 0.517 0.545 0.531 0.411 6.25 0.315 0.304 0.310 0.190 3.12 0.226 0.233 0.230 0.110 1.56 0.170 0.182 0.176 0.056 0 0.121 0.118 0.120

货期

3-5 working days