产品描述
This Human CTX- ELISA Kit was designed for the quantitative measurement of Human CTX- protein in serum, plasma, urine. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
别名
Cross Linked C-Telopeptide Of Type 2 Collagen ,CTX-2,_x000D_
Cross Linked C-Telopeptide Of Type II Collagen ,CTX-II
缩写
CTX-
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CTX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
86-98 |
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| 1:2 |
Average % |
100 |
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| Range % |
96-104 |
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| 1:4 |
Average % |
99 |
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| Range % |
92-105 |
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| 1:8 |
Average % |
92 |
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| Range % |
87-98 |
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回收率
| The recovery of human CTX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
90-100 |
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| EDTA plasma (n=4) |
100 |
95-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.462 |
2.671 |
2.567 |
2.423 |
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| 10 |
1.959 |
1.964 |
1.962 |
1.818 |
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| 5 |
1.398 |
1.345 |
1.372 |
1.228 |
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| 2.5 |
0.822 |
0.837 |
0.830 |
0.686 |
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| 1.25 |
0.546 |
0.558 |
0.552 |
0.408 |
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| 0.625 |
0.382 |
0.396 |
0.389 |
0.245 |
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| 0.312 |
0.281 |
0.297 |
0.289 |
0.145 |
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| 0 |
0.142 |
0.146 |
0.144 |
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货期
3-5 working days