产品描述
This Mouse Ifna1 ELISA Kit was designed for the quantitative measurement of Mouse Ifna1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .
别名
Ifna1 ELISA Kit; Interferon alpha-1 ELISA Kit; IFN-alpha-1 ELISA Kit
缩写
IFN-α1
Uniprot No
P01572
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-α1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 90-102 | |
| 1:2 | Average % | 90 | |
| Range % | 82-99 | |
| 1:4 | Average % | 101 | |
| Range % | 83-107 | |
| 1:8 | Average % | 98 | |
| Range % | 93-105 | |
回收率
| The recovery of mouse IFN-α1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 85-95 | |
| EDTA plasma (n=4) | 102 | 93-109 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 200 | 2.493 | 2.489 | 2.491 | 2.353 | | | 100 | 1.770 | 1.799 | 1.785 | 1.647 | | | 50 | 1.164 | 1.122 | 1.143 | 1.005 | | | 25 | 0.745 | 0.768 | 0.757 | 0.619 | | | 12.5 | 0.382 | 0.397 | 0.390 | 0.252 | | | 6.25 | 0.273 | 0.293 | 0.283 | 0.145 | | | 3.12 | 0.220 | 0.232 | 0.226 | 0.088 | | | 0 | 0.137 | 0.139 | 0.138 | | |
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货期
3-5 working days