产品描述

This Human GHRL ELISA Kit was designed for the quantitative measurement of Human GHRL protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 pg/mL-40 pg/mL and the sensitivity is 0.156 pg/mL.

别名

Appetite regulating hormone ELISA Kit; Ghrelin 27 ELISA Kit; Ghrelin 28 ELISA Kit; Ghrelin and obestatin prepropeptide ELISA Kit; Ghrelin ELISA Kit; Ghrelin/obestatin prepropeptide ELISA Kit; ghrl ELISA Kit; GHRL_HUMAN ELISA Kit; Growth hormone releasing peptide ELISA Kit; Growth hormone secretagogue ELISA Kit; Growth hormone-releasing peptide ELISA Kit; M46 protein ELISA Kit; Motilin related peptide ELISA Kit; Motilin-related peptide ELISA Kit; MTLRP ELISA Kit; Obestatin ELISA Kit; Protein M46 ELISA Kit

缩写

GHRL

Uniprot No

Q9UBU3

灵敏度

0.156 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human GHRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		86
		Range %		80-92
1:200		Average %		99
		Range %		91-105
1:400		Average %		101
		Range %		92-110
1:800		Average %		93
		Range %		86-98

回收率

The recovery of human GHRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		93		89-98
EDTA plasma (n=4)		94		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 40 2.921 2.903 2.912 2.826 20 2.405 2.348 2.383 2.297 10 1.663 1.643 1.653 1.567 5 1.053 0.989 1.021 0.935 2.5 0.575 0.603 0.589 0.503 1.25 0.322 0.319 0.321 0.235 0.625 0.214 0.229 0.222 0.136 0 0.089 0.083 0.086

货期

3-5 working days