产品描述
This Mouse DEFB1 ELISA Kit was designed for the quantitative measurement of Mouse DEFB1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
Defb1Beta-defensin 1 ELISA kit; BD-1 ELISA kit; mBD-1 ELISA kit; Defensin ELISA kit; beta 1 ELISA kit
缩写
DEFB1
Uniprot No
P56386
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Microbiology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-defensins 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
93 |
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| Range % |
89-106 |
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| 1:10 |
Average % |
92 |
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| Range % |
83-100 |
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| 1:20 |
Average % |
95 |
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| Range % |
85-110 |
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| 1:40 |
Average % |
94 |
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| Range % |
89-103 |
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回收率
| The recovery of mouse β-defensins 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
91-103 |
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| EDTA plasma (n=4) |
100 |
95-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.184 |
2.052 |
2.118 |
1.936 |
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| 500 |
1.363 |
1.377 |
1.370 |
1.188 |
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| 250 |
0.890 |
0.861 |
0.876 |
0.694 |
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| 125 |
0.517 |
0.482 |
0.500 |
0.318 |
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| 62.5 |
0.375 |
0.364 |
0.370 |
0.188 |
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| 31.2 |
0.286 |
0.267 |
0.277 |
0.095 |
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| 15.6 |
0.231 |
0.225 |
0.228 |
0.046 |
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| 0 |
0.184 |
0.179 |
0.182 |
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货期
3-5 working days