产品描述

This Human FABP5 ELISA Kit was designed for the quantitative measurement of Human FABP5 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.

别名

CFABP ELISA Kit; Cutaneous fatty acid binding protein ELISA Kit; DA11 ELISA Kit; Differentiation associated lipid binding protein LP2 ELISA Kit; E-FABP ELISA Kit; EFABP ELISA Kit; Epidermal-type fatty acid-binding protein ELISA Kit; FABP5 ELISA Kit; FABP5_HUMAN ELISA Kit; fatty acid binding protein 5 (psoriasis-associated) ELISA Kit; Fatty acid binding protein epidermal ELISA Kit; Fatty acid-binding protein 5 ELISA Kit; Fatty acid-binding protein; epidermal ELISA Kit; Keratinocyte lipid binding protein ELISA Kit; KFABP ELISA Kit; Klbp ELISA Kit; mal1 ELISA Kit; PA-FABP ELISA Kit; PAFABP ELISA Kit; Psoriasis associated fatty acid binding protein homolog ELISA Kit; Psoriasis-associated fatty acid-binding protein homolog ELISA Kit

缩写

FABP5

Uniprot No

Q01469

灵敏度

0.195 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human FABP5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		107
		Range %		94-111
1:2		Average %		98
		Range %		88-102
1:4		Average %		100
		Range %		89-104
1:8		Average %		94
		Range %		82-99

回收率

The recovery of human FABP5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		93		90-97
EDTA plasma (n=4)		100		91-104

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected 50 2.905 2.812 2.859 2.718 25 2.665 2.553 2.609 2.468 12.5 2.138 2.245 2.192 2.051 6.25 1.629 1.587 1.608 1.467 3.12 0.970 0.953 0.962 0.821 1.56 0.449 0.441 0.445 0.304 0.78 0.258 0.248 0.253 0.112 0 0.138 0.144 0.141

货期

3-5 working days