产品描述
肌酸磷酸酶B(Enolase 2),是一种酶类蛋白,参与能量代谢和糖酵解,对于细胞的生存和增殖至关重要。在某些癌症中,ENO2的过度表达与癌细胞的生长和侵袭能力有关。
华美生物所提供的Human Neuron-specific enolase,NSE ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的ENO2,其灵敏度为0.195 ng/mL,检测范围为0.78 ng/mL-50 ng/mL。
别名
2 phospho D glycerate hydrolyase ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Eno 2 ELISA Kit; ENO2 ELISA Kit; ENOG ELISA Kit; ENOG_HUMAN ELISA Kit; Enolase 2 (gamma; neuronal) ELISA Kit; Enolase 2 ELISA Kit; Enolase 2 gamma neuronal ELISA Kit; Enolase2 ELISA Kit; Epididymis secretory protein Li 279 ELISA Kit; Gamma enolase ELISA Kit; Gamma-enolase ELISA Kit; HEL S 279 ELISA Kit; Neural enolase ELISA Kit; Neuron specific enolase ELISA Kit; Neuron specific gamma enolase ELISA Kit; Neuron-specific enolase ELISA Kit; neuronal enriched enolase ELISA Kit; Neurone specific enolase ELISA Kit; NSE ELISA Kit
缩写
ENO2
Uniprot No
P09104
灵敏度
0.195 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
84-92 |
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| 1:2 |
Average % |
94 |
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| Range % |
90-98 |
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| 1:4 |
Average % |
99 |
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| Range % |
95-104 |
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| 1:8 |
Average % |
102 |
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| Range % |
96-108 |
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回收率
| The recovery of human NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
88-96 |
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| EDTA plasma (n=4) |
96 |
90-102 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 50 |
2.341 |
2.332 |
2.337 |
2.240 |
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| 25 |
1.981 |
1.978 |
1.980 |
1.883 |
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| 12.5 |
1.549 |
1.532 |
1.541 |
1.444 |
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| 6.25 |
1.014 |
0.993 |
1.004 |
0.907 |
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| 3.12 |
0.658 |
0.634 |
0.646 |
0.549 |
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| 1.56 |
0.396 |
0.382 |
0.389 |
0.292 |
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| 0.78 |
0.292 |
0.288 |
0.290 |
0.193 |
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| 0 |
0.098 |
0.095 |
0.097 |
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货期
3-5 working days