产品描述

This Human CYP27B1 ELISA Kit was designed for the quantitative measurement of Human CYP27B1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

别名

1alpha(OH)ase ELISA Kit; 25-hydroxyvitamin D(3) 1-alpha-hydroxylase ELISA Kit; 25-hydroxyvitamin D-1 alpha hydroxylase ELISA Kit; 25-OHD-1 alpha-hydroxylase ELISA Kit; Calcidiol 1-monooxygenase ELISA Kit; CP27B_HUMAN ELISA Kit; CP2B ELISA Kit; CYP1 ELISA Kit; CYP1ALPHA ELISA Kit; CYP27B ELISA Kit; Cyp27b1 ELISA Kit; Cytochrome p450 27B1 ELISA Kit; Cytochrome p450 27B13 ELISA Kit; Cytochrome P450 family 27 subfamily B polypeptide 1 ELISA Kit; Cytochrome P450 subfamily XXVIIB (25-hydroxyvitamin D-1-alpha-hydroxylase) polypeptide 1 ELISA Kit; Cytochrome P450 subfamily XXVIIB polypeptide 1 ELISA Kit; Cytochrome P450C1 alpha ELISA Kit; Cytochrome P450VD1-alpha ELISA Kit; mitochondrial ELISA Kit; P450C1 alpha ELISA Kit; P450c1 ELISA Kit; P450C1-alpha ELISA Kit; P450VD1-alpha ELISA Kit; PDDR ELISA Kit; VD3 1A hydroxylase ELISA Kit; VDD1 ELISA Kit; VDDR ELISA Kit; VDDR I ELISA Kit; VDDRI ELISA Kit; VDR ELISA Kit

缩写

CYP27B1

Uniprot No

O15528

灵敏度

7.81 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CYP27B1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		90-97
1:2		Average %		89
		Range %		85-96
1:4		Average %		96
		Range %		90-100
1:8		Average %		94
		Range %		87-97

回收率

The recovery of human CYP27B1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		85-96
EDTA plasma (n=4)		92		87-96

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2000 2.472 2.372 2.422 2.248 1000 1.715 1.615 1.665 1.491 500 1.088 1.048 1.068 0.894 250 0.711 0.701 0.706 0.532 125 0.404 0.394 0.399 0.225 62.5 0.310 0.300 0.305 0.131 31.25 0.249 0.239 0.244 0.070 0 0.175 0.173 0.174

货期

3-5 working days