产品描述

This Human GBA ELISA Kit was designed for the quantitative measurement of Human GBA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

别名

Acid beta glucosidase ELISA Kit; Acid beta-glucosidase ELISA Kit; Alglucerase ELISA Kit; Beta glucocerebrosidase ELISA Kit; BETA GLUCOSIDASE, ACID ELISA Kit; Beta-glucocerebrosidase ELISA Kit; betaGC ELISA Kit; D glucosyl N acylsphingosine glucohydrolase ELISA Kit; D-glucosyl-N-acylsphingosine glucohydrolase ELISA Kit; EC 3.2.1.45 ELISA Kit; GBA ELISA Kit; Gba protein ELISA Kit; GBA1 ELISA Kit; GC ELISA Kit; GCase ELISA Kit; GCB ELISA Kit; GLCM_HUMAN ELISA Kit; GLUC ELISA Kit; Glucocerebrosidase (alt.) ELISA Kit; Glucocerebrosidase ELISA Kit; GLUCOCEREBROSIDASE PSEUDOGENE ELISA Kit; Glucosidase beta ELISA Kit; Glucosidase, beta, acid ELISA Kit; Glucosidase, beta, acid (includes glucosylceramidase) ELISA Kit; Glucosylceramidase ELISA Kit; Imiglucerase ELISA Kit; Lysosomal glucocerebrosidase ELISA Kit; OTTHUMP00000033992 ELISA Kit; OTTHUMP00000033993 ELISA Kit

缩写

GBA

Uniprot No

P04062

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human GBA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		96
		Range %		89-100
1:2		Average %		90
		Range %		85-98
1:4		Average %		87
		Range %		83-93
1:8		Average %		92
		Range %		88-96

回收率

The recovery of human GBA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		90-103
EDTA plasma (n=4)		88		84-94

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 20 2.443 2.568 2.506 2.412 10 2.126 2.169 2.148 2.054 5 1.653 1.698 1.676 1.582 2.5 1.221 1.305 1.263 1.169 1.25 0.739 0.746 0.743 0.649 0.625 0.380 0.410 0.395 0.301 0.312 0.211 0.209 0.210 0.116 0 0.096 0.091 0.094

货期

3-5 working days