产品描述

This Human FGF2 ELISA Kit was designed for the quantitative measurement of Human FGF2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL.

别名

Basic fibroblast growth factor ELISA Kit; Basic fibroblast growth factor bFGF ELISA Kit; BFGF ELISA Kit; FGF 2 ELISA Kit; FGF B ELISA Kit; FGF-2 ELISA Kit; Fgf2 ELISA Kit; FGF2 basic ELISA Kit; FGF2_HUMAN ELISA Kit; FGFB ELISA Kit; Fibroblast growth factor 2 (basic) ELISA Kit; Fibroblast growth factor 2 ELISA Kit; Fibroblast growth factor; basic ELISA Kit; HBGF 2 ELISA Kit; HBGF-2 ELISA Kit; HBGF2 ELISA Kit; HBGH 2 ELISA Kit; HBGH2 ELISA Kit; Heparin binding growth factor 2 precursor ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; Prostatropin ELISA Kit

缩写

FGF2

Uniprot No

P09038

灵敏度

0.39 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human bFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		84
		Range %		80-92
1:2		Average %		96
		Range %		91-105
1:4		Average %		98
		Range %		93-103
1:8		Average %		93
		Range %		86-98

回收率

The recovery of human bFGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		85-98
EDTA plasma (n=4)		92		89-96

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   100 2.534 2.356 2.445 2.349   50 1.961 1.957 1.959 1.863   25 1.545 1.589 1.567 1.471   12.5 1.157 1.169 1.163 1.067   6.25 0.718 0.746 0.732 0.636   3.12 0.447 0.486 0.467 0.371   1.56 0.231 0.241 0.236 0.140   0 0.098 0.094 0.096

货期

3-5 working days