产品描述
This Rat DHEA-S ELISA Kit was designed for the quantitative measurement of Rat DHEA-S protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.
缩写
DHEA-S
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat DHEA-S in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:100 | Average % | 87 | |
| Range % | 81-92 | |
| 1:200 | Average % | 96 | |
| Range % | 90-100 | |
| 1:400 | Average % | 95 | |
| Range % | 91-104 | |
| 1:800 | Average % | 92 | |
| Range % | 84-98 | |
回收率
| The recovery of rat DHEA-S spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 85-97 | |
| EDTA plasma (n=4) | 97 | 91-101 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.103 | 2.204 | 2.154 | 2.012 | | | 1000 | 1.914 | 1.921 | 1.918 | 1.776 | | | 500 | 1.377 | 1.405 | 1.391 | 1.249 | | | 250 | 0.753 | 0.762 | 0.758 | 0.616 | | | 125 | 0.434 | 0.463 | 0.449 | 0.307 | | | 62.5 | 0.291 | 0.301 | 0.296 | 0.154 | | | 31.25 | 0.217 | 0.234 | 0.226 | 0.084 | | | 0 | 0.141 | 0.143 | 0.142 | | |
|
货期
3-5 working days