产品描述
This Mouse F1+2 ELISA Kit was designed for the quantitative measurement of Mouse F1+2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pmol/L-1000 pmol/L and the sensitivity is 3.9 pmol/L.
缩写
F1+2
灵敏度
3.9 pmol/L
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 85 | |
| Range % | 81-89 | |
| 1:2 | Average % | 109 | |
| Range % | 104-112 | |
| 1:4 | Average % | 98 | |
| Range % | 94-102 | |
| 1:8 | Average % | 92 | |
| Range % | 87-98 | |
回收率
| The recovery of mouse F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 94-99 | |
| EDTA plasma (n=4) | 91 | 87-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pmol/L | OD1 | OD2 | Average | Corrected | | | 1000 | 2.552 | 2.400 | 2.476 | 2.288 | | | 500 | 1.720 | 1.736 | 1.728 | 1.540 | | | 250 | 0.995 | 0.981 | 0.988 | 0.800 | | | 125 | 0.616 | 0.654 | 0.635 | 0.447 | | | 62.5 | 0.434 | 0.402 | 0.418 | 0.230 | | | 31.25 | 0.353 | 0.368 | 0.361 | 0.173 | | | 15.6 | 0.299 | 0.287 | 0.293 | 0.105 | | | 0 | 0.189 | 0.187 | 0.188 | | |
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货期
3-5 working days