产品描述
This Human GM-CSF Ab ELISA Kit was designed for the quantitative measurement of Human GM-CSF Ab protein in serum. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.
缩写
GM-CSF Ab
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human GM-CSF antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
85-101 |
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| 1:2 |
Average % |
96 |
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| Range % |
84-100 |
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| 1:4 |
Average % |
96 |
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| Range % |
90-103 |
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| 1:8 |
Average % |
95 |
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| Range % |
82-106 |
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回收率
| The recovery of human GM-CSF antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
86-101 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
2.597 |
2.648 |
2.623 |
2.518 |
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| 20 |
2.123 |
2.131 |
2.127 |
2.022 |
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| 10 |
1.466 |
1.472 |
1.469 |
1.364 |
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| 5 |
0.831 |
0.896 |
0.864 |
0.759 |
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| 2.5 |
0.472 |
0.494 |
0.483 |
0.378 |
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| 1.25 |
0.312 |
0.297 |
0.305 |
0.200 |
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| 0.625 |
0.225 |
0.241 |
0.233 |
0.128 |
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| 0 |
0.102 |
0.107 |
0.105 |
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货期
3-5 working days