产品描述

This Human FADD ELISA Kit was designed for the quantitative measurement of Human FADD protein in serum, plasma, cell culture supernates, cell lysates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.

别名

FADD ELISA Kit; FADD protein ELISA Kit; FADD_HUMAN ELISA Kit; Fas (TNFRSF6) associated via death domain ELISA Kit; Fas associated via death domain ELISA Kit; Fas associating death domain containing protein ELISA Kit; Fas associating protein ELISA Kit; Fas associating protein with death domain ELISA Kit; Fas TNFRSF6 associated via death domain ELISA Kit; FAS-associated death domain protein ELISA Kit; FAS-associating death domain-containing protein ELISA Kit; GIG 3 ELISA Kit; GIG3 ELISA Kit; Growth inhibiting gene 3 protein ELISA Kit; Growth-inhibiting gene 3 protein ELISA Kit; H sapiens mRNA for mediator of receptor induced toxicity ELISA Kit; Mediator of receptor induced toxicity ELISA Kit; MGC8528 ELISA Kit; MORT 1 ELISA Kit; MORT1 ELISA Kit; Protein FADD ELISA Kit

缩写

FADD

Uniprot No

Q13158

灵敏度

31.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of Human FADD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		88-96
1:2		Average %		97
		Range %		92-103
1:4		Average %		94
		Range %		89-98
1:8		Average %		98
		Range %		92-104

回收率

The recovery of Human FADD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		88		82-94
EDTA plasma (n=4)		98		92-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 8000 2.703 2.822 2.763 2.659 4000 2.328 2.208 2.268 2.164 2000 1.615 1.534 1.575 1.471 1000 0.884 0.914 0.899 0.795 500 0.433 0.468 0.451 0.347 250 0.281 0.294 0.288 0.184 125 0.182 0.186 0.184 0.080 0 0.104 0.103 0.104

货期

3-5 working days