产品描述
The Rat Vascular Endothelial cell Growth Factor D (VEGF-D) ELISA kit is a powerful tool for researchers studying the cardiovascular system in rats. VEGF-D is a protein known for its role in promoting the growth and survival of blood vessels, making it an important target for cardiovascular research.
Our ELISA kit utilizes a quantitative sandwich assay principle to measure the levels of VEGF-D in serum, plasma, and tissue homogenates with a detection range of 0.312 ng/mL to 20 ng/mL and a sensitivity of 0.078 ng/mL. With an assay time of only 1-5 hours and a sample volume of 50-100ul, this kit is both efficient and convenient for researchers.
Whether you are investigating the mechanisms of angiogenesis and lymphatic regeneration or studying the effects of cardiovascular disease on the vasculature, this Rat Vascular Endothelial cell Growth Factor D (VEGF-D) ELISA kit is the perfect tool for your research needs.
别名
Vegfd ELISA kit; FigfVascular endothelial growth factor D ELISA kit; VEGF-D ELISA kit; c-Fos-induced growth factor ELISA kit; FIGF ELISA kit
缩写
FIGF
Uniprot No
O35251
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGF-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 87 | | |
| Range % | 83-92 | | |
| 1:2 | Average % | 101 | | |
| Range % | 97-105 | | |
| 1:4 | Average % | 91 | | |
| Range % | 85-97 | | |
| 1:8 | Average % | 97 | | |
| Range % | 91-103 | | |
回收率
| The recovery of rat VEGF-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
|
| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 91 | 86-95 | | |
| EDTA plasma (n=4) | 98 | 94-102 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | | 20 | 1.932 | 1.837 | 1.885 | 1.738 | | | | 10 | 1.456 | 1.566 | 1.511 | 1.364 | | | | 5 | 1.088 | 1.042 | 1.065 | 0.918 | | | | 2.5 | 0.713 | 0.707 | 0.710 | 0.563 | | | | 1.25 | 0.492 | 0.481 | 0.487 | 0.340 | | | | 0.625 | 0.358 | 0.342 | 0.350 | 0.203 | | | | 0.312 | 0.272 | 0.278 | 0.275 | 0.128 | | | | 0 | 0.148 | 0.146 | 0.147 | | | |
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货期
3-5 working days
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