产品描述
CUSABIO's Kim-1 mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Kim-1 in serum, plasma, tissue homogenates, or urine. This assay uses an antibody specific for mouse Kim-1 coated on a 96-well plate. Kim-1 present in a sample is bound to the wells by the immobilized antibody after adding the sample into the wells. Biotinylated Kim-1 antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB substrate solution is added to the wells and color develops in proportion to the amount of Kim-1 bound. The addition of Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a microplate reader.
Kim-1 (HAVCR1) is most highly upregulated in the proximal tubular epithelium in acute and chronic kidney injury. Kim-1 functions as a phosphatidylserine receptor that recognizes and internalizes apoptotic cells and also acts as a scavenger receptor that mediates the uptake of modified low-density lipoprotein and necrotic-cell debris. A large amount of Kim-1 protein is also released into the urine, making it a useful urinary biomarker for kidney injury. In the immune system, Kim-1 is also involved in activating the differentiation of Th1, Th2, and Th17. And Kim-1 is also considered as an activating receptor in B cells, dendritic cells, and natural killer T cells.
别名
Havcr1 ELISA Kit; Kim1 ELISA Kit; Tim1 ELISA Kit; Timd1 ELISA Kit; Hepatitis A virus cellular receptor 1 homolog ELISA Kit; HAVcr-1 ELISA Kit; Kidney injury molecule 1 ELISA Kit; KIM-1 ELISA Kit; T cell immunoglobulin and mucin domain-containing protein 1 ELISA Kit; TIMD-1 ELISA Kit; T cell membrane protein 1 ELISA Kit; T-cell immunoglobulin mucin receptor 1 ELISA Kit; TIM-1 ELISA Kit; CD antigen CD365 ELISA Kit
缩写
HAVCR1
Uniprot No
Q5QNS5
灵敏度
0.225 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Microbiology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Kim-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 93 | |
| Range % | 87-99 | |
| 1:2 | Average % | 92 | |
| Range % | 88-98 | |
| 1:4 | Average % | 96 | |
| Range % | 90-101 | |
| 1:8 | Average % | 95 | |
| Range % | 92-99 | |
回收率
| The recovery of mouse Kim-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 89-97 | |
| EDTA plasma (n=4) | 94 | 88-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 60 | 2.356 | 2.345 | 2.351 | 2.183 | | | 30 | 1.786 | 1.725 | 1.756 | 1.588 | | | 15 | 1.264 | 1.236 | 1.250 | 1.082 | | | 7.5 | 0.813 | 0.801 | 0.807 | 0.639 | | | 3.75 | 0.513 | 0.489 | 0.501 | 0.333 | | | 1.8 | 0.378 | 0.363 | 0.371 | 0.203 | | | 0.9 | 0.267 | 0.251 | 0.259 | 0.091 | | | 0 | 0.170 | 0.166 | 0.168 | | |
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货期
3-5 working days
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