产品描述
This Chicken IGF1 ELISA Kit was designed for the quantitative measurement of Chicken IGF1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 125 pg/mL-2000 pg/mL and the sensitivity is 125 pg/mL.
别名
IGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
缩写
IGF1
Uniprot No
P18254
灵敏度
125 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
86-96 |
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| 1:2 |
Average % |
97 |
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| Range % |
92-105 |
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| 1:4 |
Average % |
87 |
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| Range % |
85-90 |
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| 1:8 |
Average % |
96 |
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| Range % |
93-99 |
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回收率
| The recovery of chicken IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
85-94 |
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| EDTA plasma (n=4) |
95 |
90-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 2000 |
0.183 |
0.194 |
0.189 |
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| 1000 |
0.311 |
0.333 |
0.322 |
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| 500 |
0.526 |
0.500 |
0.513 |
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| 250 |
1.024 |
1.028 |
1.026 |
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| 125 |
2.107 |
2.120 |
2.114 |
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货期
3-5 working days