产品描述
This Mouse CXCL14 ELISA Kit was designed for the quantitative measurement of Mouse CXCL14 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
别名
Cxcl14 ELISA Kit; Bmac ELISA Kit; Kec ELISA Kit; Ks1 ELISA Kit; Mip2g ELISA Kit; Scyb14C-X-C motif chemokine 14 ELISA Kit; B-cell and monocyte-activating chemokine ELISA Kit; Chemokine BRAK ELISA Kit; Kidney-expressed chemokine CXC ELISA Kit; MIP-2G ELISA Kit; Small-inducible cytokine B14 ELISA Kit
缩写
CXCL14
Uniprot No
Q9WUQ5
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CXCL14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 84 | | |
| Range % | 80-92 | | |
| 1:2 | Average % | 103 | | |
| Range % | 99-108 | | |
| 1:4 | Average % | 97 | | |
| Range % | 92-101 | | |
| 1:8 | Average % | 89 | | |
| Range % | 84-93 | | |
回收率
| The recovery of mouse CXCL14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 95 | 90-100 | | |
| EDTA plasma (n=4) | 97 | 92-101 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | | 20 | 2.737 | 2.652 | 2.695 | 2.547 | | | | 10 | 2.235 | 2.276 | 2.256 | 2.108 | | | | 5 | 1.533 | 1.604 | 1.569 | 1.421 | | | | 2.5 | 0.915 | 0.938 | 0.927 | 0.779 | | | | 1.25 | 0.557 | 0.524 | 0.541 | 0.393 | | | | 0.625 | 0.312 | 0.327 | 0.320 | 0.172 | | | | 0.312 | 0.239 | 0.229 | 0.234 | 0.086 | | | | 0 | 0.146 | 0.150 | 0.148 | | | |
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货期
3-5 working days