产品描述

This Human GREM1 ELISA Kit was designed for the quantitative measurement of Human GREM1 protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

别名

BMP antagonist 1 ELISA Kit; Cell proliferation inducing gene 2 protein ELISA Kit; Cell proliferation-inducing gene 2 protein ELISA Kit; CKTSF1B1 ELISA Kit; colorectal adenoma and carcinoma 1 ELISA Kit; Cysteine knot superfamily 1 ELISA Kit; Cysteine knot superfamily 1; BMP antagonist 1 ELISA Kit; Cysteine knot superfamily BMP antagonist 1 ELISA Kit; DAN domain family member 2 ELISA Kit; DAND2 ELISA Kit; Down regulated in Mos-transformed cells protein ELISA Kit; Down-regulated in Mos-transformed cells protein ELISA Kit; DRM ELISA Kit; grem1 ELISA Kit; GREM1_HUMAN ELISA Kit; Gremlin 1 homolog; cysteine knot superfamily ELISA Kit; Gremlin 1 like protein ELISA Kit; Gremlin 1; cysteine knot superfamily; homolog ELISA Kit; GREMLIN ELISA Kit; Gremlin-1 ELISA Kit; Gremlin1 ELISA Kit; IHG-2 ELISA Kit; IHG2 ELISA Kit; Increased in high glucose 2 ELISA Kit; Increased in high glucose protein 2 ELISA Kit; PIG2 ELISA Kit; Proliferation inducing gene 2 ELISA Kit; proliferation inducing gene 2 protein ELISA Kit

缩写

GREM1

Uniprot No

O60565

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human GREM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		87-99
1:2		Average %		100
		Range %		95-106
1:4		Average %		89
		Range %		85-92
1:8		Average %		90
		Range %		85-99

回收率

The recovery of human GREM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		84-96
EDTA plasma (n=4)		87		82-96

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.487 2.586 2.537 2.404 5 2.285 2.296 2.291 2.158 2.5 1.803 1.923 1.863 1.730 1.25 1.371 1.432 1.402 1.269 0.625 0.869 0.889 0.879 0.746 0.312 0.581 0.587 0.584 0.451 0.156 0.176 0.184 0.180 0.047 0 0.132 0.134 0.133

货期

3-5 working days