产品描述

This Human FLNA ELISA Kit was designed for the quantitative measurement of Human FLNA protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.68 pg/mL.

别名

ABP 280 ELISA Kit; ABP-280 ELISA Kit; Actin-binding protein 280 ELISA Kit; Alpha filamin ELISA Kit; Alpha-filamin ELISA Kit; APBX ELISA Kit; CSBS ELISA Kit; CVD1 ELISA Kit; Endothelial actin binding protein ELISA Kit; Endothelial actin-binding protein ELISA Kit; Filamin 1 ELISA Kit; Filamin A alpha ELISA Kit; Filamin A ELISA Kit; Filamin-1 ELISA Kit; Filamin-A ELISA Kit; FLN ELISA Kit; FLN-A ELISA Kit; FLN1 ELISA Kit; FLNA ELISA Kit; FLNA_HUMAN ELISA Kit; FMD ELISA Kit; MNS ELISA Kit; NHBP ELISA Kit; Non muscle filamin ELISA Kit; Non-muscle filamin ELISA Kit; OPD ELISA Kit; OPD1 ELISA Kit; OPD2 ELISA Kit; XLVD ELISA Kit; XMVD ELISA Kit

缩写

FLNA

Uniprot No

P21333

灵敏度

4.68 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human FLNA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		95
		Range %		91-104
1:10		Average %		89
		Range %		87-93
1:20		Average %		98
		Range %		93-102
1:40		Average %		90
		Range %		87-94

回收率

The recovery of human FLNA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		92-101
EDTA plasma (n=4)		98		90-104

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1200 2.592 2.492 2.542 2.401 600 2.012 1.912 1.962 1.821 300 1.383 1.283 1.333 1.192 150 0.729 0.719 0.724 0.583 75 0.436 0.426 0.431 0.290 37.5 0.344 0.334 0.339 0.198 18.75 0.228 0.218 0.223 0.082 0 0.142 0.140 0.141

货期

3-5 working days