产品描述
This Goat IgA ELISA Kit was designed for the quantitative measurement of Goat IgA protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 29 ng/mL-7500 ng/mL and the sensitivity is 29 ng/mL.
缩写
IgA
灵敏度
29 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of goat immunoglobulin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5000 |
Average % |
94 |
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| Range % |
90-103 |
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| 1:10000 |
Average % |
96 |
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| Range % |
92-100 |
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| 1:20000 |
Average % |
90 |
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| Range % |
85-97 |
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| 1:40000 |
Average % |
92 |
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| Range % |
85-103 |
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回收率
| The recovery of goat immunoglobulin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
90-100 |
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| EDTA plasma (n=4) |
95 |
91-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 7500 |
0.289 |
0.312 |
0.301 |
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| 1875 |
0.558 |
0.542 |
0.550 |
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| 468 |
0.912 |
0.921 |
0.917 |
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| 117 |
1.612 |
1.543 |
1.578 |
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| 29 |
1.853 |
1.934 |
1.894 |
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| 0 |
2.406 |
2.398 |
2.402 |
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货期
3-5 working days