产品描述
This Rat Hsp90aa1 ELISA Kit was designed for the quantitative measurement of Rat Hsp90aa1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
别名
Hsp90aa1 ELISA Kit; Hsp86 ELISA Kit; HspcaHeat shock protein HSP 90-alpha ELISA Kit; Heat shock 86 kDa ELISA Kit; HSP 86 ELISA Kit; HSP86 ELISA Kit
缩写
HSP-90
Uniprot No
P82995
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Hsp90aa1/Hsp86/Hspca in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 87-97 | |
| 1:2 | Average % | 96 | |
| Range % | 89-103 | |
| 1:4 | Average % | 102 | |
| Range % | 98-106 | |
| 1:8 | Average % | 87 | |
| Range % | 81-95 | |
回收率
| The recovery of rat Hsp90aa1/Hsp86/Hspca spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 87-94 | |
| EDTA plasma (n=4) | 101 | 97-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.517 | 2.735 | 2.626 | 2.433 | | | 10 | 1.772 | 1.859 | 1.816 | 1.623 | | | 5 | 1.159 | 1.164 | 1.162 | 0.969 | | | 2.5 | 0.680 | 0.740 | 0.710 | 0.517 | | | 1.25 | 0.517 | 0.539 | 0.528 | 0.335 | | | 0.625 | 0.374 | 0.388 | 0.381 | 0.188 | | | 0.312 | 0.290 | 0.314 | 0.302 | 0.109 | | | 0 | 0.189 | 0.197 | 0.193 | | |
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货期
3-5 working days