产品描述
This Rat ENO1 ELISA Kit was designed for the quantitative measurement of Rat ENO1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.
别名
Eno1 ELISA Kit; Eno-1Alpha-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 1 ELISA Kit; Non-neural enolase ELISA Kit; NNE ELISA Kit
缩写
ENO1
Uniprot No
P04764
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat α-enolase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 93 | | |
| Range % | 88-97 | | |
| 1:2 | Average % | 102 | | |
| Range % | 97-107 | | |
| 1:4 | Average % | 101 | | |
| Range % | 95-105 | | |
| 1:8 | Average % | 94 | | |
| Range % | 91-98 | | |
回收率
| The recovery of rat α-enolase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 105 | 99-108 | | |
| EDTA plasma (n=4) | 87 | 84-92 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | | 40 | 2.191 | 2.275 | 2.233 | 2.111 | | | | 20 | 1.448 | 1.538 | 1.493 | 1.371 | | | | 10 | 0.921 | 0.963 | 0.942 | 0.820 | | | | 5 | 0.563 | 0.572 | 0.568 | 0.446 | | | | 2.5 | 0.334 | 0.341 | 0.338 | 0.216 | | | | 1.25 | 0.231 | 0.245 | 0.238 | 0.116 | | | | 0.625 | 0.173 | 0.179 | 0.176 | 0.054 | | | | 0 | 0.121 | 0.122 | 0.122 | | | |
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货期
3-5 working days