产品描述
The product CSB-E04545m is a sandwich ELISA kit developed to measure concentrations of mouse FASL in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of FASL in samples. The color intensity is measured at 450 nm via a microplate reader.
FASL is well known for its ability to deliver a death signal through its receptor Fas. FASL is expressed in various types of cancer and is involved in tumor immune evasion. The Fas-FASL system plays an important role in the establishment of immune privilege status for tumors by inducing Fas-mediated apoptosis in tumor-specific lymphocytes. However, studies have demonstrated that high levels of FASL trigger tumor rejection and that FASL promotes tumor growth at low levels possibly by inhibiting anti-tumor immune responses.
别名
Faslg; Apt1lg1; Cd95l; Fasl; gld; Tnfsf6; Tumor necrosis factor ligand superfamily member 6; CD95 ligand; CD95-L; Fas antigen ligand; Fas ligand; FasL; CD antigen CD178
缩写
FASLG
Uniprot No
P41047
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FASL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 86 | | |
| Range % | 82-91 | | |
| 1:2 | Average % | 101 | | |
| Range % | 98-105 | | |
| 1:4 | Average % | 87 | | |
| Range % | 84-91 | | |
| 1:8 | Average % | 100 | | |
| Range % | 95-105 | | |
回收率
| The recovery of mouse FASL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 89 | 85-94 | | |
| EDTA plasma (n=4) | 93 | 90-96 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | | 500 | 2.411 | 2.431 | 2.421 | 2.234 | | | | 250 | 1.846 | 1.801 | 1.824 | 1.637 | | | | 125 | 1.181 | 1.201 | 1.191 | 1.004 | | | | 62.5 | 0.824 | 0.831 | 0.828 | 0.641 | | | | 31.2 | 0.531 | 0.562 | 0.547 | 0.360 | | | | 15.6 | 0.358 | 0.348 | 0.353 | 0.166 | | | | 7.8 | 0.271 | 0.280 | 0.276 | 0.089 | | | | 0 | 0.188 | 0.186 | 0.187 | | | |
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货期
3-5 working days
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