产品描述
CUSABIO's GLP-1 human ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GLP-1 in serum, plasma, or tissue homogenates. This assay uses an antibody specific to human GLP-1 coated on a 96-well plate. GLP-1 present in a sample is bound to the wells by the immobilized antibody after adding the sample into the wells. Biotinylated GLP-1 antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB substrate solution is added to the wells and color develops in proportion to the amount of GLP-1 bound. The addition of Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a microplate reader.
GLP-1 is a gastrointestinal peptide that is produced by the enteroendocrine L cells and is released in response to meal intake. GLP-1 mainly functions to stimulate insulin secretion and to suppress food intake, gastric emptying, and glucagon secretion, thereby limiting postprandial glucose excursions. It is important for glucose homeostasis. It also inhibits gastrointestinal motility and secretion and thus acts as an enterogastrone and part of the "ileal brake" mechanism. GLP-1 stimulates the proliferation, differentiation, and neogenesis of β cells.
别名
N/A
缩写
GLP-1
灵敏度
0.5 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human GLP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
87-107 |
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| 1:2 |
Average % |
96 |
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| Range % |
90-106 |
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| 1:4 |
Average % |
95 |
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| Range % |
86-105 |
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| 1:8 |
Average % |
96 |
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| Range % |
89-100 |
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回收率
| The recovery of human GLP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
91-101 |
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| EDTA plasma (n=4) |
95 |
89-103 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
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| 2.3 |
0.063 |
0.061 |
0.062 |
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| 4.6 |
0.169 |
0.172 |
0.171 |
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| 9.1 |
0.398 |
0.384 |
0.391 |
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| 22.9 |
0.861 |
0.872 |
0.867 |
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| 40 |
1.660 |
1.653 |
1.657 |
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货期
3-5 working days
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