产品描述
This Human DEFB104A ELISA Kit was designed for the quantitative measurement of Human DEFB104A protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL .
别名
BD 4 ELISA Kit; BD-4 ELISA Kit; BD4 ELISA Kit; beta 104 ELISA Kit; beta 4 Defensin ELISA Kit; Beta defensin 4 ELISA Kit; Beta-defensin 104 ELISA Kit; Beta-defensin 4 ELISA Kit; D104A_HUMAN ELISA Kit; DEFB-4 ELISA Kit; DEFB104B ELISA Kit; Defensin ELISA Kit; Defensin beta 104 ELISA Kit; Defensin beta 4 precursor ELISA Kit; hBD 4 ELISA Kit; hBD-4 ELISA Kit; hBD4 ELISA Kit
缩写
DEFB104A
Uniprot No
Q8WTQ1
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human DEFB104A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 91 | |
| Range % | 86-97 | |
| 1:2 | Average % | 92 | |
| Range % | 87-100 | |
| 1:4 | Average % | 85 | |
| Range % | 81-93 | |
| 1:8 | Average % | 92 | |
| Range % | 86-98 | |
回收率
| The recovery of human DEFB104A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 89 | 84-96 | |
| EDTA plasma (n=4) | 92 | 85-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.706 | 2.655 | 2.681 | 2.525 | | | 1000 | 2.136 | 2.011 | 2.074 | 1.918 | | | 500 | 1.346 | 1.289 | 1.318 | 1.162 | | | 250 | 0.764 | 0.752 | 0.758 | 0.602 | | | 125 | 0.367 | 0.354 | 0.361 | 0.205 | | | 62.5 | 0.279 | 0.287 | 0.283 | 0.127 | | | 31.25 | 0.244 | 0.231 | 0.238 | 0.082 | | | 0 | 0.162 | 0.150 | 0.156 | | |
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货期
3-5 working days