产品描述

The human GOT2 ELISA Kit is engineered for accurate measurement of human GOT2 levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the human GOT2 content in the sample. The color intensity is positively correlated with GOT2 content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.

GOT2 is a mitochondrial glutamate oxaloacetate transaminase that catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). It facilitates the cellular uptake of long-chain free fatty acids. GOT2 plays a key role in amino acid metabolism, intracellular NAD(H) redox balance, as well as metabolite exchange between mitochondria and cytosol. Studies have shown that loss of GOT2 disturbs redox homeostasis and halts the proliferation of pancreatic ductal adenocarcinoma (PDA) cells in vitro.

别名

AATM_HUMAN ELISA Kit; AL022787 ELISA Kit; Aspartate aminotransferase 2 ELISA Kit; Aspartate aminotransferase ELISA Kit; Aspartate aminotransferase; mitochondrial ELISA Kit; Aspartate transaminase 2 ELISA Kit; ASPATA ELISA Kit; EC 2.6.1.1 ELISA Kit; FABP 1 ELISA Kit; FABP pm ELISA Kit; FABP-1 ELISA Kit; FABPpm ELISA Kit; Fatty acid binding protein ELISA Kit; Fatty acid-binding protein ELISA Kit; FLJ40994 ELISA Kit; Glutamate oxaloacetate transaminase 2 ELISA Kit; Glutamate oxaloacetate transaminase 2; mitochondrial ELISA Kit; Glutamate oxaloacetate transaminase; mitochondrial ELISA Kit; Glutamic oxaloacetic transaminase 2; mitochondrial (aspartate aminotransferase 2) ELISA Kit; Got 2 ELISA Kit; GOT2 ELISA Kit; KAT4 ELISA Kit; KATIV ELISA Kit; kynurenine aminotransferase 4 ELISA Kit; Kynurenine aminotransferase IV ELISA Kit; kynurenine--oxoglutarate transaminase 4 ELISA Kit; kynurenine--oxoglutarate transaminase IV ELISA Kit; mAspAT ELISA Kit; MGC102129 ELISA Kit; MGC115763 ELISA Kit; mitAAT ELISA Kit; mitochondrial ELISA Kit; Mitochondrial aspartate aminotransferase ELISA Kit; OTTMUSP00000017748 ELISA Kit; Plasma membrane fatty acid binding protein ELISA Kit; Plasma membrane-associated fatty acid-binding protein ELISA Kit; Transaminase A ELISA Kit

缩写

GOT2

Uniprot No

P00505

灵敏度

0.039 mU/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human GOT2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		85
		Range %		80-89
1:2		Average %		92
		Range %		88-97
1:4		Average %		96
		Range %		91-100
1:8		Average %		87
		Range %		82-91

回收率

The recovery of human GOT2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		103		98-109
EDTA plasma (n=4)		95		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average Corrected 10 2.186 2.268 2.227 2.028 5 1.765 1.794 1.780 1.581 2.5 1.391 1.380 1.386 1.187 1.25 0.928 0.911 0.920 0.721 0.625 0.654 0.623 0.639 0.440 0.312 0.439 0.459 0.449 0.250 0.156 0.327 0.341 0.334 0.135 0 0.202 0.195 0.199

货期

3-5 working days