产品描述
This Human FGF13 ELISA Kit was designed for the quantitative measurement of Human FGF13 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.
别名
FGF 13 ELISA Kit; FGF 2 ELISA Kit; FGF-13 ELISA Kit; FGF13 ELISA Kit; FGF13_HUMAN ELISA Kit; FGF2 ELISA Kit; FHF 2 ELISA Kit; FHF-2 ELISA Kit; FHF2 ELISA Kit; Fibroblast growth factor 13 ELISA Kit; Fibroblast growth factor homologous factor 2 ELISA Kit; OTTHUMP00000024143 ELISA Kit; OTTHUMP00000024144 ELISA Kit
缩写
FGF13
Uniprot No
Q92913
灵敏度
3.12 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human FGF-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 85 | | |
| Range % | 80-90 | | |
| 1:2 | Average % | 96 | | |
| Range % | 92-100 | | |
| 1:4 | Average % | 90 | | |
| Range % | 85-94 | | |
| 1:8 | Average % | 92 | | |
| Range % | 88-96 | | |
回收率
| The recovery of human FGF-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 88 | 84-92 | | |
| EDTA plasma (n=4) | 104 | 100-109 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 800 | 2.152 | 2.263 | 2.208 | 2.062 | | | | 400 | 1.607 | 1.712 | 1.660 | 1.514 | | | | 200 | 1.179 | 1.096 | 1.138 | 0.992 | | | | 100 | 0.781 | 0.745 | 0.763 | 0.617 | | | | 50 | 0.435 | 0.451 | 0.443 | 0.297 | | | | 25 | 0.314 | 0.307 | 0.311 | 0.165 | | | | 12.5 | 0.228 | 0.234 | 0.231 | 0.085 | | | | 0 | 0.143 | 0.149 | 0.146 | | | |
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货期
3-5 working days