产品描述
This Human IAPP ELISA Kit was designed for the quantitative measurement of Human IAPP protein in serum, plasma, urine. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.75 pg/mL.
别名
Amylin ELISA Kit; DAP ELISA Kit; Diabetes associated peptide ELISA Kit; Diabetes-associated peptide ELISA Kit; IAP ELISA Kit; IAPP ELISA Kit; IAPP_HUMAN ELISA Kit; Insulinoma amyloid peptide ELISA Kit; Islet amyloid polypeptide (diabetes associated peptide, amylin) ELISA Kit; Islet amyloid polypeptide ELISA Kit
缩写
IAPP
Uniprot No
P10997
灵敏度
11.75 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays):CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human IAPP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 83 | | |
| Range % | 80-86 | | |
| 1:2 | Average % | 102 | | |
| Range % | 98-106 | | |
| 1:4 | Average % | 93 | | |
| Range % | 90-96 | | |
| 1:8 | Average % | 105 | | |
| Range % | 101-109 | | |
回收率
| The recovery of human IAPP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 89 | 85-93 | | |
| EDTA plasma (n=4) | 102 | 98-106 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | | 3000 | 1.901 | 1.932 | 1.917 | 1.774 | | | | 1500 | 1.547 | 1.527 | 1.537 | 1.394 | | | | 750 | 1.125 | 1.136 | 1.131 | 0.988 | | | | 375 | 0.798 | 0.778 | 0.788 | 0.645 | | | | 187.5 | 0.537 | 0.528 | 0.533 | 0.390 | | | | 94 | 0.326 | 0.312 | 0.319 | 0.176 | | | | 47 | 0.244 | 0.241 | 0.243 | 0.100 | | | | 0 | 0.145 | 0.140 | 0.143 | | | |
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货期
3-5 working days