产品描述
This Rat HTR2A ELISA Kit was designed for the quantitative measurement of Rat HTR2A protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
别名
Htr2a; Htr2; 5-hydroxytryptamine receptor 2A; 5-HT-2; 5-HT-2A; Serotonin receptor 2A
缩写
HTR2A
Uniprot No
P14842
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat 5-HT-2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 82-91 | |
| 1:2 | Average % | 104 | |
| Range % | 98-110 | |
| 1:4 | Average % | 90 | |
| Range % | 85-94 | |
| 1:8 | Average % | 84 | |
| Range % | 80-88 | |
回收率
| The recovery of rat 5-HT-2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 89 | 84-96 | |
| EDTA plasma (n=4) | 95 | 90-103 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.121 | 2.095 | 2.108 | 2.004 | | | 1000 | 1.874 | 1.858 | 1.866 | 1.762 | | | 500 | 1.546 | 1.533 | 1.540 | 1.436 | | | 250 | 1.120 | 1.102 | 1.111 | 1.007 | | | 125 | 0.707 | 0.718 | 0.713 | 0.609 | | | 62.5 | 0.498 | 0.516 | 0.507 | 0.403 | | | 31.25 | 0.295 | 0.280 | 0.288 | 0.184 | | | 0 | 0.104 | 0.103 | 0.104 | | |
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货期
3-5 working days