产品描述
The Chicken Immunoglobulin A (IgA) ELISA Kit can be your reliable solution for measuring immunoglobulin A in Gallus gallus (chicken) samples. This ELISA kit is designed to detect and quantify IgA protein in serum and plasma samples with a wide detection range of 781.25 ng/mL-50000 ng/mL and a sensitivity of 390 ng/mL.
This kit utilizes a competitive measurement principle, making it a highly quantitative assay for researchers in the field of immunology. The assay time is quick and efficient, taking only 1-5 hours to complete, and requires a small sample volume of 50-100ul. The detection wavelength of 450 nm ensures high accuracy and precision in your measurements.
With over 17 citations, our Chicken Immunoglobulin A (IgA) ELISA Kit has been tested and validated by numerous researchers, providing accurate and reliable results in their studies. It is an essential tool for researchers studying the immune system of chickens, allowing them to better understand the mechanisms and functions of IgA protein in this species.
缩写
IgA
灵敏度
390 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:100 | Average % | 97 | |
| Range % | 93-105 | |
| 1:200 | Average % | 94 | |
| Range % | 91-98 | |
| 1:400 | Average % | 102 | |
| Range % | 98-106 | |
| 1:800 | Average % | 88 | |
| Range % | 82-96 | |
回收率
| The recovery of chicken IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 92-102 | |
| EDTA plasma (n=4) | 102 | 98-108 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | | | | 50000 | 0.072 | 0.071 | 0.072 | | | | 25000 | 0.134 | 0.136 | 0.135 | | | | 12500 | 0.231 | 0.242 | 0.237 | | | | 6250 | 0.426 | 0.435 | 0.431 | | | | 3125 | 0.666 | 0.675 | 0.671 | | | | 1562.5 | 0.938 | 0.927 | 0.933 | | | | 781.25 | 1.248 | 1.147 | 1.198 | | | | 0 | 1.695 | 1.594 | 1.645 | | |
|
货期
3-5 working days
已关闭
固定模块
收藏
易享客服
