产品描述

This Human ENTPD1 ELISA Kit was designed for the quantitative measurement of Human ENTPD1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.44 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.

别名

ATPDase ELISA Kit; CD 39 ELISA Kit; CD39 ELISA Kit; CD39 antigen ELISA Kit; DKFZp686D194 ELISA Kit; DKFZp686I093 ELISA Kit; Ecto apyrase ELISA Kit; Ecto ATP diphosphohydrolase ELISA Kit; Ecto-apyrase ELISA Kit; Ecto-ATP diphosphohydrolase 1 ELISA Kit; Ecto-ATPase 1 ELISA Kit; Ecto-ATPDase 1 ELISA Kit; Ectonucleoside triphosphate diphosphohydrolase 1 ELISA Kit; ENTP1_HUMAN ELISA Kit; ENTPD 1 ELISA Kit; ENTPD1 ELISA Kit; FLJ40921 ELISA Kit; FLJ40959 ELISA Kit; Lymphoid cell activation antigen ELISA Kit; NTPDase 1 ELISA Kit; NTPDase1 ELISA Kit; SPG64 ELISA Kit

缩写

ENTPD1

Uniprot No

P49961

灵敏度

5.86 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ENTPD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		84
		Range %		81-89
1:2		Average %		97
		Range %		93-102
1:4		Average %		84
		Range %		80-88
1:8		Average %		99
		Range %		95-102

回收率

The recovery of human ENTPD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		100		97-103
EDTA plasma (n=4)		107		103-110

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1500 2.161 2.343 2.252 2.059 750 1.724 1.708 1.716 1.523 375 1.296 1.274 1.285 1.092 187.5 0.891 0.885 0.888 0.695 93.75 0.562 0.598 0.580 0.387 46.88 0.401 0.421 0.411 0.218 23.44 0.287 0.289 0.288 0.095 0 0.191 0.194 0.193

货期

3-5 working days