产品描述

This Human DLL4 ELISA Kit was designed for the quantitative measurement of Human DLL4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

别名

AOS6 ELISA Kit; Delta 4 ELISA Kit; delta 4 precursor ELISA Kit; Delta ligand 4 ELISA Kit; delta ligand 4 precursor ELISA Kit; Delta like 4 ELISA Kit; Delta like 4 homolog ELISA Kit; Delta like 4 protein ELISA Kit; Delta like canonical Notch ligand 4 ELISA Kit; Delta like protein 4 ELISA Kit; Delta-like 4 (Drosophila) ELISA Kit; Delta-like protein 4 ELISA Kit; Delta4 ELISA Kit; DLL 4 ELISA Kit; Dll4 ELISA Kit; DLL4_HUMAN ELISA Kit; Drosophila Delta homolog 4 ELISA Kit; hdelta2 ELISA Kit; Homeobox protein DLL-4 ELISA Kit; MGC126344 ELISA Kit; Notch ligand delta 2 ELISA Kit; Notch ligand DLL4 ELISA Kit; Notch ligand DLL4 precursor ELISA Kit; XDLL-4 ELISA Kit

缩写

DLL4

Uniprot No

Q9NR61

灵敏度

5.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Developmental Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human DLL4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		84
		Range %		81-89
1:2		Average %		94
		Range %		90-98
1:4		Average %		83
		Range %		80-86
1:8		Average %		87
		Range %		83-90

回收率

The recovery of human DLL4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		91-99
EDTA plasma (n=4)		100		96-104

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1500 2.261 2.298 2.280 2.098 750 1.575 1.595 1.585 1.403 375 0.989 0.975 0.982 0.800 187.5 0.643 0.606 0.625 0.443 94 0.434 0.429 0.432 0.250 47 0.317 0.338 0.328 0.146 23.5 0.267 0.278 0.273 0.091 0 0.181 0.183 0.182

货期

3-5 working days