产品描述

This Human ENPP3 ELISA Kit was designed for the quantitative measurement of Human ENPP3 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

Alkaline phosphodiesterase I ELISA Kit; ARHR2 ELISA Kit; B10 ELISA Kit; CD203c ELISA Kit; CD203c antigen ELISA Kit; dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) ELISA Kit; dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) ELISA Kit; E NPP 3 ELISA Kit; E-NPP 3 ELISA Kit; Ectonucleotide pyrophosphatase/phosphodiesterase 3 ELISA Kit; Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 ELISA Kit; ENPP3 ELISA Kit; ENPP3_HUMAN ELISA Kit; gp130RB13 6 ELISA Kit; gp130RB136 ELISA Kit; M6S1 ELISA Kit; NPP1 ELISA Kit; NPP3 ELISA Kit; NPPase ELISA Kit; NPPS ELISA Kit; Nucleotide pyrophosphatase ELISA Kit; PC 1 ELISA Kit; PC-1 ELISA Kit; PCA1 ELISA Kit; PD Ibeta ELISA Kit; PD-Ibeta ELISA Kit; PDNP1 ELISA Kit; PDNP3 ELISA Kit; Phosphodiesterase I beta ELISA Kit; Phosphodiesterase I/nucleotide pyrophosphatase 3 ELISA Kit; phosphodiesterase i/nucleotide pyrophosphatase beta ELISA Kit

缩写

ENPP3

Uniprot No

O14638

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ENPP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		97
		Range %		93-101
1:2		Average %		99
		Range %		92-105
1:4		Average %		89
		Range %		83-94
1:8		Average %		91
		Range %		87-96

回收率

The recovery of human ENPP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		104		98-109
EDTA plasma (n=4)		85		80-90

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected 10 2.755 2.858 2.807 2.691 5 2.212 2.221 2.217 2.101 2.5 1.537 1.477 1.507 1.391 1.25 0.946 0.954 0.950 0.834 0.625 0.489 0.473 0.481 0.365 0.312 0.289 0.274 0.282 0.166 0.156 0.210 0.218 0.214 0.098 0 0.115 0.116 0.116

货期

3-5 working days