华美生物/CUSABIOHIF1A大鼠低氧诱导因子1α(HIF-1α)酶联免疫试剂盒Rat hypoxia-inducible factor 1α,HIF-1α ELISA KitCSB-E08540r华美-易享

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HIF1A大鼠低氧诱导因子1α(HIF-1α)酶联免疫试剂盒Rat hypoxia-inducible factor 1α,HIF-1α ELISA Kit

数万篇文献的选择，荣登各大SCI高分期刊

原料试剂研发实验室

价格

￥2500.00-3900.00

品牌华美生物/CUSABIO

地区中国,湖北省,武汉市

货号 CSB-E08540r

产地国产

选择规格

96T

48T

易享客服

武汉华美生物工程有限公司

湖北省武汉东湖新技术开发区神墩五路269号生之源股份生物科创产业园B栋

营业执照已审核

武汉华美生物工程有限公司成立于2007年12月，是一家以科研试剂生产、诊断试剂原料供应为核心，集自主研发、生产、营销为一体的生物高新技术企业。华美生物拥有多项先进的核心技术，致力于为全球高等院校、政府研究机构、诊断试剂生产厂家以及生物制药公司提供高质量产品与高水平的技术服务。华美生物子品牌CUSABIO主要经营科研试剂产品，现拥有各物种基因产品一万余种；现货重组蛋白16,000多种；数十个种属的多克隆抗体65,000多种和千余种高质量单克隆抗体。ELISA试剂盒现已涵盖20多个种属、3,000个靶标、4,000多种产品，涉及肿瘤、激素、自身免疫、心血管、代谢等众多研究领域。华美CUSABIO产品远销欧美日德等九十多个国家和地区，已被众多国际顶级学术期刊Nature, Cell, Immunity, Nature Medicine, Cell Metabolism, Gastroenterology等引用。华美生物总部位于湖北武汉，在美国建立了子公司，并在全球近百个国家拥有授权经销商。华美生物始终秉承“诚信与品质”的核心理念，借助自身的创新实力，匠心打造精品科研试剂与诊断原料，无畏前行，尽情展现着开创者的梦想与未来，为人类健康事业创造价值！

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产品规格

品牌名称

华美生物/CUSABIO

货号

CSB-E08540r

国产/进口

国产

规格

96T,48T

图文详情

This Rat HIF1A ELISA Kit was designed for the quantitative measurement of Rat HIF1A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .

Hif1aHypoxia-inducible factor 1-alpha ELISA Kit; HIF-1-alpha ELISA Kit; HIF1-alpha ELISA Kit

HIF1A

O35800

0.78 pg/mL

1-5h

50-100ul

450 nm

Epigenetics and Nuclear Signaling

quantitative

To assess the linearity of the assay, samples were spiked with high concentrations of rat HIF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-94
1:2	Average %	96
	Range %	91-100
1:4	Average %	93
	Range %	87-97
1:8	Average %	100
	Range %	96-103

The recovery of rat HIF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-95
EDTA plasma (n=4)	95	88-98

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.715	2.656	2.686	2.538
100	1.730	1.676	1.703	1.555
50	1.196	1.179	1.188	1.040
25	0.608	0.618	0.613	0.465
12.5	0.404	0.412	0.408	0.260
6.25	0.303	0.316	0.310	0.162
3.12	0.248	0.237	0.243	0.095
0	0.150	0.146	0.148

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

3-5 working days

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