产品描述
The mouse IgE ELISA Kit allows for the in vitro quantitative determination of IgE concentrations in serum, plasma, or tissue homogenates. This kit exclusively recognizes mouse IgE. IgE is only found in mammals and is the least abundant immunoglobulin isotype in circulation. IgE is renowned for its functions in Th2 responses, protection against helminth parasites, and pathogenesis of allergy. IgE signals by binding to the high-affinity receptor FcεR1 and the low-affinity receptor FcεR2 or CD23. It plays an important role in acute allergic reactions and chronic inflammatory allergic diseases such as allergic rhinitis. IgE-FcεR1 signaling promotes inflammation and vascular remodeling. Type 1 hypersensitivity involves IgE-mediated activation of mast cells or basophils and subsequent release of preformed mediators including histamine, leukotrienes, and prostaglandins into the environment, finally causing the characteristic symptoms of allergy.
The detection mechanism of this kit is based on the Sandwich-ELISA technique. IgE in the sample is bound to the capture antibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled IgE antibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with IgE content in the sample. The kit has been quality-controlled with high sensitivity, strong specificity, good linearity, high precision, high recovery, and high lot-to-lot consistency.
缩写
IgE
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 93 | |
| Range % | 89-95 | |
| 1:2 | Average % | 102 | |
| Range % | 99-105 | |
| 1:4 | Average % | 93 | |
| Range % | 87-96 | |
| 1:8 | Average % | 99 | |
| Range % | 94-101 | |
回收率
| The recovery of mouse IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 91-97 | |
| EDTA plasma (n=4) | 95 | 91-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.435 | 2.328 | 2.382 | 2.245 | | | 50 | 1.704 | 1.684 | 1.694 | 1.557 | | | 25 | 1.182 | 1.166 | 1.174 | 1.037 | | | 12.5 | 0.577 | 0.590 | 0.584 | 0.447 | | | 6.25 | 0.400 | 0.410 | 0.405 | 0.268 | | | 3.12 | 0.307 | 0.292 | 0.300 | 0.163 | | | 1.56 | 0.258 | 0.248 | 0.253 | 0.116 | | | 0 | 0.138 | 0.136 | 0.137 | | |
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货期
3-5 working days
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