产品描述

This Human HMGCR ELISA Kit was designed for the quantitative measurement of Human HMGCR protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL- 50 ng/mL and the sensitivity is 0.195 ng/mL .

别名

3 hydroxy 3 methylglutaryl CoA reductase ELISA Kit; 3 hydroxy 3 methylglutaryl Coenzyme A reductase ELISA Kit; 3 hydroxymethylglutaryl CoA reductase ELISA Kit; 3-hydroxy-3-methylglutaryl CoA reductase (NADPH) ELISA Kit; 3-hydroxy-3-methylglutaryl-coenzyme A reductase ELISA Kit; 3H3M ELISA Kit; HMDH_HUMAN ELISA Kit; HMG CoA reductase ELISA Kit; HMG CoAR ELISA Kit; HMG-CoA reductase ELISA Kit; Hmgcr ELISA Kit; Hydroxymethylglutaryl CoA reductase ELISA Kit; LDLCQ3 ELISA Kit; MGC103269 ELISA Kit; Red ELISA Kit

缩写

HMGCR

Uniprot No

P04035

灵敏度

0.195 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human HMG-CoAR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		95
		Range %		92-100
1:2		Average %		91
		Range %		82-96
1:4		Average %		95
		Range %		87-100
1:8		Average %		87
		Range %		82-98

回收率

The recovery of HMG-CoAR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		91-101
EDTA plasma (n=4)		90		82-97

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 50 2.325 2.450 2.388 2.271 25 2.050 2.174 2.112 1.995 12.5 1.476 1.563 1.520 1.403 6.25 0.659 0.697 0.678 0.561 3.12 0.245 0.261 0.253 0.136 1.56 0.192 0.186 0.189 0.072 0.78 0.151 0.140 0.146 0.029 0 0.120 0.113 0.117

货期

3-5 working days