产品描述
This Pig IFNA1 ELISA Kit was designed for the quantitative measurement of Pig IFNA1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL .
缩写
IFN-α
Uniprot No
Q6VAB8
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 89 | |
| Range % | 80-99 | |
| 1:2 | Average % | 98 | |
| Range % | 91-105 | |
| 1:4 | Average % | 96 | |
| Range % | 92-105 | |
| 1:8 | Average % | 94 | |
| Range % | 86-98 | |
回收率
| The recovery of pig IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-102 | |
| EDTA plasma (n=4) | 97 | 90-100 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.895 | 2.852 | 2.874 | 2.739 | | | 250 | 2.114 | 2.117 | 2.116 | 1.981 | | | 125 | 1.254 | 1.260 | 1.257 | 1.122 | | | 62.5 | 0.846 | 0.839 | 0.843 | 0.708 | | | 31.2 | 0.489 | 0.454 | 0.472 | 0.337 | | | 15.6 | 0.301 | 0.319 | 0.310 | 0.175 | | | 7.8 | 0.257 | 0.249 | 0.253 | 0.118 | | | 0 | 0.133 | 0.137 | 0.135 | | |
|
货期
3-5 working days