产品描述
表皮素(Epinephrine)是由肾上腺髓质细胞合成的一种儿茶酚胺类激素,也称为肾上腺素。在应激时,表皮素能够激活心血管系统、增加血糖和血脂、扩张气道等。它是应激反应的重要调节因子。表皮素在人体内的浓度随着应激而升高。在应激反应中,表皮素释放后能够迅速刺激骨髓中的造血干细胞,增加白细胞和红细胞的数量,提高机体的应对能力。
华美生物所提供的Rat Epinephrine/Adrenaline,EPI ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测大鼠血清、血浆、组织匀浆样本中的EPI,其灵敏度为0.5 pg/mL,检测范围为1 pg/mL-200 pg/mL。
缩写
EPI
灵敏度
0.5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat EPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
98 |
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| Range % |
90-103 |
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| 1:2 |
Average % |
90 |
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| Range % |
85-96 |
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| 1:4 |
Average % |
94 |
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| Range % |
89-99 |
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| 1:8 |
Average % |
85 |
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| Range % |
80-90 |
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回收率
| The recovery of rat EPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
91 |
85-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 200 |
0.203 |
0.202 |
0.203 |
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| 50 |
0.378 |
0.351 |
0.365 |
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| 16 |
0.581 |
0.537 |
0.559 |
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| 4 |
0.945 |
1.012 |
0.979 |
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| 1 |
1.416 |
1.493 |
1.455 |
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| 0 |
2.241 |
2.367 |
2.304 |
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货期
3-5 working days