产品描述

This Human EIF2C2 ELISA Kit was designed for the quantitative measurement of Human EIF2C2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

别名

Ago 2 ELISA Kit; AGO2_HUMAN ELISA Kit; Argonaute 2 ELISA Kit; argonaute 2; RISC catalytic component ELISA Kit; Argonaute RISC catalytic component 2 ELISA Kit; Argonaute2 ELISA Kit; CTA-204B4.6 ELISA Kit; dAgo2 ELISA Kit; eIF 2C 2 ELISA Kit; eIF-2C 2 ELISA Kit; eIF2C 2 ELISA Kit; Eif2c2 ELISA Kit; Eukaryotic translation initiation factor 2C 2 ELISA Kit; Eukaryotic translation initiation factor 2C subunit 2 ELISA Kit; hAgo2 ELISA Kit; MGC3183 ELISA Kit; PAZ Piwi domain protein ELISA Kit; PPD ELISA Kit; Protein argonaute-2 ELISA Kit; Protein slicer ELISA Kit; Q10 ELISA Kit; Slicer protein ELISA Kit

缩写

EIF2C2

Uniprot No

Q9UKV8

灵敏度

6.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human EIF2C2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		95
		Range %		92-99
1:2		Average %		97
		Range %		93-102
1:4		Average %		94
		Range %		89-98
1:8		Average %		88
		Range %		84-93

回收率

The recovery of human EIF2C2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		99		94-105
EDTA plasma (n=4)		86		82-92

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1600 2.197 2.325 2.261 2.115 800 1.387 1.486 1.437 1.291 400 0.833 0.799 0.816 0.670 200 0.495 0.507 0.501 0.355 100 0.304 0.316 0.310 0.164 50 0.242 0.231 0.237 0.091 25 0.188 0.184 0.186 0.040 0 0.145 0.146 0.146

货期

3-5 working days