产品描述
This Duck IFN- ELISA Kit was designed for the quantitative measurement of Duck IFN- protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写
IFN-
Uniprot No
Q9YGB9
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of duck IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
86-98 |
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| 1:2 |
Average % |
98 |
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| Range % |
92-106 |
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| 1:4 |
Average % |
85 |
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| Range % |
81-86 |
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| 1:8 |
Average % |
88 |
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| Range % |
83-94 |
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回收率
| The recovery of duck IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
87-94 |
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| EDTA plasma (n=4) |
103 |
101-107 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.235 |
2.199 |
2.217 |
2.072 |
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| 500 |
1.528 |
1.655 |
1.592 |
1.447 |
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| 250 |
1.187 |
1.120 |
1.154 |
1.009 |
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| 125 |
0.734 |
0.786 |
0.760 |
0.615 |
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| 62.5 |
0.555 |
0.550 |
0.553 |
0.408 |
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| 31.2 |
0.346 |
0.324 |
0.335 |
0.190 |
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| 15.6 |
0.204 |
0.208 |
0.206 |
0.061 |
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| 0 |
0.140 |
0.149 |
0.145 |
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货期
3-5 working days