产品描述

This Human DEFB103A ELISA Kit was designed for the quantitative measurement of Human DEFB103A protein in serum, plasma, urine, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL .

别名

BD 3 ELISA Kit; BD-3 ELISA Kit; BD3 ELISA Kit; beta 103 ELISA Kit; Beta defensin 103A ELISA Kit; Beta defensin 103A precursor ELISA Kit; Beta defensin 3 ELISA Kit; Beta-defensin 103 ELISA Kit; Beta-defensin 3 ELISA Kit; D103A_HUMAN ELISA Kit; DEF B3 ELISA Kit; DEFB 103 ELISA Kit; DEFB 103A ELISA Kit; DEFB 3 ELISA Kit; DEFB-3 ELISA Kit; DEFB103 ELISA Kit; DEFB103A ELISA Kit; DEFB103B ELISA Kit; DEFB3 ELISA Kit; Defensin ELISA Kit; Defensin beta 103A ELISA Kit; Defensin beta 3 ELISA Kit; Defensin like protein ELISA Kit; Defensin-like protein ELISA Kit; HBD 3 ELISA Kit; hBD-3 ELISA Kit; HBD3 ELISA Kit; HBP 3 ELISA Kit; HBP3 ELISA Kit; mBD3 ELISA Kit

缩写

DEFB103A

Uniprot No

P81534

灵敏度

31.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human β-defensins 3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		96
		Range %		91-101
1:2		Average %		98
		Range %		92-104
1:4		Average %		92
		Range %		87-96
1:8		Average %		90
		Range %		86-94

回收率

The recovery of human β-defensins 3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		87		82-94
EDTA plasma (n=4)		102		98-106

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 8000 2.339 2.508 2.424 2.270 4000 1.833 1.869 1.851 1.697 2000 1.185 1.198 1.192 1.038 1000 0.699 0.687 0.693 0.539 500 0.375 0.392 0.384 0.230 250 0.294 0.281 0.288 0.134 125 0.208 0.211 0.210 0.056 0 0.153 0.155 0.154

货期

3-5 working days