产品描述

This Human CYP24A1 ELISA Kit was designed for the quantitative measurement of Human CYP24A1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

别名

1 25 @dihydroxyvitamin D3 24 hydroxylase ELISA Kit; 1 25 dihydroxyvitamin D(3) 24 hydroxylase ELISA Kit; 1 ELISA Kit; 1,25-dihydroxyvitamin D(3) 24-hydroxylase; mitochondrial ELISA Kit; 24 OHase ELISA Kit; 24-OHase ELISA Kit; 25-dihydroxyvitamin D(3) 24-hydroxylase ELISA Kit; CP 24 ELISA Kit; CP24 ELISA Kit; CP24A_HUMAN ELISA Kit; CYP 24 ELISA Kit; CYP24 ELISA Kit; CYP24A1 ELISA Kit; Cytochrome P450 24A1 ELISA Kit; Cytochrome P450 24A1 mitochondrial ELISA Kit; cytochrome P450 CC24 ELISA Kit; Cytochrome P450 family 24 ELISA Kit; Cytochrome P450 family 24 subfamily A member 1 ELISA Kit; Cytochrome P450 family 24 subfamily A polypeptide 1 ELISA Kit; Cytochrome P450 subfamily XXIV ELISA Kit; Cytochrome P450; subfamily XXIV (vitamin D 24-hydroxylase) ELISA Kit; Cytochrome P450-CC24 ELISA Kit; EC 1.14.13.n4 ELISA Kit; Exo mitochondrial protein ELISA Kit; HCAI ELISA Kit; HCINF1 ELISA Kit; MGC126273 ELISA Kit; MGC126274 ELISA Kit; mitochondrial ELISA Kit; P450 CC24 ELISA Kit; Vitamin D 24 hydroxylase ELISA Kit; Vitamin D(3) 24 hydroxylase ELISA Kit; Vitamin D(3) 24-hydroxylase ELISA Kit

缩写

CYP24A1

Uniprot No

Q07973

灵敏度

1.95 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CYP24A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		86-97
1:2		Average %		89
		Range %		83-94
1:4		Average %		97
		Range %		91-102
1:8		Average %		98
		Range %		92-103

回收率

The recovery of human CYP24A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		88-99
EDTA plasma (n=4)		86		80-91

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 500 2.719 2.612 2.666 2.534 250 2.226 2.121 2.174 2.042 125 1.407 1.504 1.456 1.324 62.5 0.845 0.821 0.833 0.701 31.2 0.457 0.466 0.462 0.330 15.6 0.323 0.314 0.319 0.187 7.8 0.221 0.226 0.224 0.092 0 0.133 0.131 0.132

货期

3-5 working days