产品描述
This Dog EDN1 ELISA Kit was designed for the quantitative measurement of Dog EDN1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.
别名
EDN1Endothelin-1 ELISA kit; ET-1 ELISA kit; Preproendothelin-1 ELISA kit; PPET1) [Cleaved into: Big endothelin-1] ELISA kit
缩写
EDN1
Uniprot No
P13206
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of dog ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 85-96 | |
| 1:2 | Average % | 94 | |
| Range % | 90-99 | |
| 1:4 | Average % | 89 | |
| Range % | 81-96 | |
| 1:8 | Average % | 100 | |
| Range % | 91-108 | |
回收率
| The recovery of dog ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 87 | 82-96 | |
| EDTA plasma (n=4) | 97 | 82-106 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 200 | 2.954 | 2.989 | 2.972 | 2.861 | | | 100 | 2.388 | 2.398 | 2.393 | 2.282 | | | 50 | 1.758 | 1.789 | 1.774 | 1.663 | | | 25 | 1.035 | 1.069 | 1.052 | 0.941 | | | 12.5 | 0.502 | 0.543 | 0.523 | 0.412 | | | 6.25 | 0.271 | 0.268 | 0.270 | 0.159 | | | 3.12 | 0.184 | 0.169 | 0.177 | 0.066 | | | 0 | 0.112 | 0.110 | 0.111 | | |
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货期
3-5 working days