产品描述
This Human FERMT3 ELISA Kit was designed for the quantitative measurement of Human FERMT3 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名
Fermitin family homolog 3 ELISA Kit; Fermitin family member 3 ELISA Kit; FERMT3 ELISA Kit; Kind3 ELISA Kit; Kindlin 3 ELISA Kit; Kindlin-3 ELISA Kit; Kindlin3 ELISA Kit; MGC10966 ELISA Kit; MIG 2 ELISA Kit; MIG2 like protein ELISA Kit; MIG2-like protein ELISA Kit; MIG2B ELISA Kit; Unc 112 related protein 2 ELISA Kit; Unc-112-related protein 2 ELISA Kit; UNC112C ELISA Kit; URP2 ELISA Kit; URP2_HUMAN ELISA Kit; URP2SF ELISA Kit
缩写
FERMT3
Uniprot No
Q86UX7
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human FERMT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 88 | |
| Range % | 82-94 | |
| 1:2 | Average % | 89 | |
| Range % | 85-95 | |
| 1:4 | Average % | 94 | |
| Range % | 86-98 | |
| 1:8 | Average % | 92 | |
| Range % | 88-96 | |
回收率
| The recovery of human FERMT3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 102 | 97-105 | |
| EDTA plasma (n=4) | 98 | 92-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.447 | 2.295 | 2.371 | 2.269 | | | 5 | 1.718 | 1.810 | 1.764 | 1.662 | | | 2.5 | 1.287 | 1.216 | 1.252 | 1.150 | | | 1.25 | 0.847 | 0.862 | 0.855 | 0.753 | | | 0.625 | 0.543 | 0.537 | 0.540 | 0.438 | | | 0.312 | 0.343 | 0.355 | 0.349 | 0.247 | | | 0.156 | 0.222 | 0.214 | 0.218 | 0.116 | | | 0 | 0.103 | 0.101 | 0.102 | | |
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货期
3-5 working days