产品描述
This Chicken IFN-β/IFNB ELISA Kit was designed for the quantitative measurement of Chicken IFN-β/IFNB protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 2.5 pg/mL-200 pg/mL and the sensitivity is 3.125 pg/mL.
别名
IFNB ELISA Kit; IFN2 ELISA Kit; Interferon type B ELISA Kit; IFN-beta ELISA Kit
缩写
IFN-β/IFNB
Uniprot No
Q90873
灵敏度
3.125 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
99 |
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| Range % |
95-103 |
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| 1:2 |
Average % |
94 |
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| Range % |
88-98 |
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| 1:4 |
Average % |
102 |
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| Range % |
98-106 |
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| 1:8 |
Average % |
89 |
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| Range % |
84-96 |
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回收率
| The recovery of chicken IFN-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
88-94 |
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| EDTA plasma (n=4) |
88 |
82-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 200 |
0.342 |
0.341 |
0.342 |
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| 75 |
0.494 |
0.486 |
0.490 |
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| 37.5 |
0.652 |
0.664 |
0.658 |
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| 12.5 |
1.067 |
1.076 |
1.072 |
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| 2.5 |
1.478 |
1.575 |
1.527 |
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| 0 |
1.854 |
1.955 |
1.905 |
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货期
3-5 working days