产品描述
二肽基肽酶-4(Dipeptidyl peptidase-4,DPP4)是一种细胞膜结合型蛋白酶,能够剪切细胞表面的多肽并参与多种生理过程。DPP4在肠道中能够切割胰高血糖素样肽-1(GLP-1)和胰岛素样生长因子-2(IGF-2),从而调节肠道吸收葡萄糖和胰岛素的分泌。DPP4还参与了免疫调节、炎症反应、细胞增殖和转移等生理和病理过程。DPP4的功能异常与多种疾病相关,如糖尿病、肥胖、心血管疾病、癌症等。因此,DPP4成为了潜在的药物靶点,DPP4抑制剂已经成功地用于治疗2型糖尿病。
华美生物所提供的Human dipeptidyl peptidase Ⅳ,DPPⅣ ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的DPP4,其灵敏度为0.39 ng/mL,检测范围为1.56 ng/mL-100 ng/mL。
别名
DPP4; ADCP2; CD26; Dipeptidyl peptidase 4; ADABP; Adenosine deaminase complexing protein 2; ADCP-2; Dipeptidyl peptidase IV; DPP IV; T-cell activation antigen CD26; TP103; CD antigen CD26
缩写
DPP4
Uniprot No
P27487
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human DPPⅣ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
92-102 |
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| 1:2 |
Average % |
89 |
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| Range % |
83-98 |
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| 1:4 |
Average % |
94 |
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| Range % |
87-102 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-105 |
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回收率
| The recovery of human DPPⅣ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
83-106 |
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| EDTA plasma (n=4) |
89 |
85-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
2.931 |
2.852 |
2.892 |
2.773 |
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| 50 |
2.438 |
2.495 |
2.467 |
2.348 |
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| 25 |
1.703 |
1.762 |
1.733 |
1.614 |
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| 12.5 |
0.943 |
0.975 |
0.959 |
0.840 |
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| 6.25 |
0.536 |
0.560 |
0.548 |
0.429 |
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| 3.12 |
0.332 |
0.325 |
0.329 |
0.210 |
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| 1.56 |
0.228 |
0.236 |
0.232 |
0.113 |
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| 0 |
0.118 |
0.120 |
0.119 |
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货期
3-5 working days