产品描述
维生素B9,又名叶酸或者叶酸酯,是一种水溶性维生素。它在DNA合成、细胞生长和分裂过程中起重要作用,也是红细胞生成和神经系统发育所必需的物质。缺乏维生素B9可能导致巨幼红细胞贫血和神经系统疾病等健康问题。
华美生物所提供的Mouse Folic acid,FA ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测鼠血清、血浆样本中的FA,其灵敏度为0.195 ng/mL,检测范围为0.195 ng/mL-50 ng/mL。
别名
N/A
缩写
FA
灵敏度
0.195 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
97 |
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| Range % |
90-105 |
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| 1:2 |
Average % |
89 |
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| Range % |
83-96 |
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| 1:4 |
Average % |
92 |
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| Range % |
84-99 |
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| 1:8 |
Average % |
91 |
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| Range % |
85-99 |
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回收率
| The recovery of mouse FA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
87-100 |
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| EDTA plasma (n=4) |
95 |
89-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
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| 50 |
0.321 |
0.332 |
0.327 |
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| 12.5 |
0.512 |
0.537 |
0.525 |
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| 3.12 |
0.826 |
0.851 |
0.839 |
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| 0.78 |
1.175 |
1.153 |
1.164 |
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| 0.195 |
1.655 |
1.632 |
1.644 |
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| 0 |
1.985 |
1.948 |
1.967 |
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货期
3-5 working days