产品描述
This Rabbit F1+2 ELISA Kit was designed for the quantitative measurement of Rabbit F1+2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 pmol/mL-20 pmol/mL and the sensitivity is 0.078 pmol/mL.
缩写
F1+2
灵敏度
0.078 pmol/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
80-88 |
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| 1:2 |
Average % |
98 |
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| Range % |
92-104 |
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| 1:4 |
Average % |
97 |
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| Range % |
93-101 |
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| 1:8 |
Average % |
87 |
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| Range % |
82-95 |
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回收率
| The recovery of rabbit F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
86 |
83-92 |
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| EDTA plasma (n=4) |
92 |
85-97 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pmol/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.989 |
2.788 |
2.889 |
2.786 |
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| 10 |
2.535 |
2.372 |
2.454 |
2.351 |
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| 5 |
1.781 |
1.815 |
1.798 |
1.695 |
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| 2.5 |
1.002 |
1.012 |
1.007 |
0.904 |
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| 1.25 |
0.485 |
0.506 |
0.496 |
0.393 |
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| 0.625 |
0.301 |
0.311 |
0.306 |
0.203 |
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| 0.312 |
0.178 |
0.175 |
0.177 |
0.074 |
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| 0 |
0.103 |
0.102 |
0.103 |
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货期
3-5 working days