产品描述
VEGFR-3/Flt-4是一种血管内皮生长因子受体家族成员,参与血管生成和淋巴管生成等过程。VEGFR-3/Flt-4的功能异常与许多疾病有关,如心血管疾病、肿瘤和淋巴水肿等。
华美生物所提供的Human Vascuar endothelial cell growth factor receptor 3,VEGFR-3/Flt-4 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的VEGFR-3/Flt-4,其灵敏度为0.039 ng/mL,检测范围为0.156 ng/mL-10 ng/mL。
别名
EC 2.7.10.1 ELISA Kit; flt 4 ELISA Kit; FLT-4 ELISA Kit; FLT4 ELISA Kit; FLT41 ELISA Kit; Fms related tyrosine kinase 4 ELISA Kit; Fms-like tyrosine kinase 4 ELISA Kit; LMPH1A ELISA Kit; PCL ELISA Kit; Soluble VEGFR3 variant 1 ELISA Kit; Soluble VEGFR3 variant 2 ELISA Kit; Soluble VEGFR3 variant 3 ELISA Kit; Tyrosine protein kinase receptor FLT4 ELISA Kit; Tyrosine-protein kinase receptor FLT4 ELISA Kit; Vascular endothelial growth factor receptor 3 ELISA Kit; Vascular endothelial growth factor receptor 3 precursor ELISA Kit; VEGF R3 ELISA Kit; VEGFR 3 ELISA Kit; VEGFR-3 ELISA Kit; VEGFR3 ELISA Kit; VGFR3_HUMAN ELISA Kit
缩写
FLT4
Uniprot No
P35916
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human VEGFR-3/Flt-44 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
98 |
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| Range % |
91-105 |
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| 1:2 |
Average % |
90 |
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| Range % |
85-96 |
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| 1:4 |
Average % |
95 |
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| Range % |
85-105 |
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| 1:8 |
Average % |
92 |
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| Range % |
83-98 |
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回收率
| The recovery of human VEGFR-3/Flt-44 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
86-100 |
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| EDTA plasma (n=4) |
93 |
87-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.549 |
2.516 |
2.533 |
2.420 |
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| 5 |
1.517 |
1.557 |
1.537 |
1.424 |
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| 2.5 |
0.796 |
0.758 |
0.777 |
0.664 |
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| 1.25 |
0.472 |
0.489 |
0.481 |
0.368 |
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| 0.625 |
0.302 |
0.315 |
0.309 |
0.196 |
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| 0.312 |
0.259 |
0.246 |
0.253 |
0.140 |
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| 0.156 |
0.164 |
0.171 |
0.168 |
0.055 |
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| 0 |
0.111 |
0.115 |
0.113 |
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货期
3-5 working days